In vitro fungicidal effect of the floral extract of Cattleya walkeriana (Gardner, 1839) (Orchidaceae)

Authors

DOI:

https://doi.org/10.14295/bjs.v4i7.761

Keywords:

Cattleya genus, Colletotrichum acutatum, Aspergillus flavus, phytochemistry, antibiosis

Abstract

Some orchid groups have shown promising results regarding biological activity in inhibiting fungal growth. This study aimed to evaluate the antifungal activity of the hydroethanolic floral extract of Cattleya walkeriana against strains of Sclerotinia sclerotiorum, Colletotrichum acutatum, C. gloeosporioides, and Aspergillus spp. (A. flavus, A. niger, and A. fumigatus) at different concentrations through in vitro assays. The extract was prepared using a 70% (v/v) hydroethanolic solution and tested on Petri dishes containing potato dextrose agar (PDA) medium inoculated with fungal strains. Results were expressed in millimeters (mm) of fungal growth inhibition (antibiosis) at concentrations of 100%, 80%, 50%, 10%, and 1.25% (v/v). The study demonstrated that higher concentrations (80% and 100%) of the hydroethanolic floral extract of Cattleya walkeriana exhibited strong antifungal activity against the tested fungal strains.

References

Barros, F., Vinhos, F., Rodrigues, V. T., Barberene, F. F. V. A., & Fraga, C. N. (2010). Orchidaceae: Lista de Espécies da Flora do Brasil. Jardim Botânico do Rio de Janeiro. 2010. Available on <http://floradobrasil.jbrj.gov.br/2010/FB000179>. Accessed on June 10, 2025.

Faria, R. T., Santiago, D. C., Saridakis, D. P., Albino, U. B., & Araújo, R. (2002). Preservation of the brazilian orchid Cattleya walkeriana Gardner using in vitro propagation. Crop Breeding and Applied Biotechnology, 2(3), 489-492.

Ferreira, D. F. (2019). SISVAR: A computer analysis system to fixed effects split plot type designs. Brazilian Journal of Biometrics, 37(4), 529-535. https://doi.org/10.28951/rbb.v37i4.450

Garcia, R. À., Juliatti, F. C., Barbosa, A. G., & Cassemiro, T. A. (2012). Atividade antifúngica de óleo e extratos vegetais sobre Sclerotinia sclerotiorum. Bioscience Journal, 28(1), 48-57. https://seer.ufu.br/index.php/biosciencejournal/article/view/8174

Houch, P. R., & Mendes, M. A. (1999). Cattleya walkeriana. O Mundo das Orquídeas, 2, 13-15.

Irimescu, L-S., Diguţă, C. F., Encea, R. Ş., & Matei, F. (2020). Preliminary study on the antimicrobial potential of Phalaenopsis orchids methanolic extracts. Scientific Bulletin, Series F. Biotechnologies, XXIV(2), 149-153.

Kumari, R., Shakula, K., & Debasish, S. (2017). Extraction and screening of bioactive compounds of some common hydrophytic and wetland plants from East Singbhum. IOSR Journal of Pharmacy, 7(11), 23-29.

Menezes Filho, A. C. P., Castro, C. F. S., Silva, A. P., & Cruz, R. M. (2021). Avaliação físico-química, fitoquímica e atividades biológicas do extrato hidroetanólico floral de Spathoglotis unguiculata (Labill.) Rchb. f. (Orchidaceae). Arquivos Científicos, 4(1), 79-87. https://arqcientificosimmes.emnuvens.com.br/abi/article/view/509

Menezes Filho, A. C. P., & Castro, C. F. S. (2019). Análise fitoquímica preliminar de extratos foliares de orchidaceas (Cattleya walkeriana Gardner), (Oncidium cebolleta Sw.), (Encyclia linearifolioides Kraenzl.) e (Polystachya concreta (Jacq.) Garay & H. R. Sweet. Revista Ensaios e Ciência, 23(10, 16-23. https://doi.org/10.17921/1415-6938.2019v23n1p16-23

Miller, D., & Warren, R. (1996). O gênero Catleya Lindl. p. 256. In: Orquídeas do Alto da Serra. Lis, São Paulo.

Nardelli, M. S., Viana, A., Montiel, C. B., Kuhn, S. B., Liesenfeld, V., & Fortes, A. M. T. Desenvolvimento de plântulas de Cattleya walkeriana Gardner em diferentes meios de cultura. Journal of Agronomic Sciences, 9(1), 61-72.

Saikia, J., & Thakur, D. (2024). A review on endophytic bacteria of orchids: functional roles toward synthesis of bioactive metabolites for plant growth promotion and disease biocontrol. Planta, 260. https://doi.org/10.1007/s00425-024-04501-3

Silva, C. I., & Milaneze-Gutierre, M. A. (2004). Caracterização morfo-anatômica dos órgãos vegetativos de Cattleya walkeriana Gardner (Orchidaceae). Acta Scientiarum. Biological Sciences, 26(1), 91-100.

Schneiders, D., Pescador, R., Booz, M. R., & Suzuki, R. M. (2012). Germinação crescimento e desenvolvimento in vitro de orquídeas (Cattleya spp, Orchidaceae). Revista Ceres, 59(2), 185-191.

Downloads

Published

2025-06-19

How to Cite

Moreira, C. A. C., Barros, T. H. L., Rocha, E. N. da, Ventura, M. V. A., & Menezes Filho, A. C. P. de. (2025). In vitro fungicidal effect of the floral extract of Cattleya walkeriana (Gardner, 1839) (Orchidaceae). Brazilian Journal of Science, 4(7), 23–28. https://doi.org/10.14295/bjs.v4i7.761

Issue

Section

Agrarian and Biological Sciences