Phytochemical and physicochemical evaluation, and photoprotection, antioxidant, antifungal, and antibacterial activities of the floral extract of Schubertia grandiflora Mart. & Zucc. (Apocynaceae)
DOI:
https://doi.org/10.14295/bjs.v1i1.4Keywords:
Phytomolecules, Candida genus, Staphylococcus aureus, Schubertia genusAbstract
Schubertia grandiflora is a species belonging to the floral of the Cerrado domain, presented annually with aromatic flowers. The study aimed to evaluate the hydroethanolic floral extract of S. grandiflora for phytochemistry, physicochemical and photoprotection, antioxidant, antifungal and antibacterial activities. Fresh flowers were collected and the floral extract produced by maceration. Qualitative phytochemical tests were carried out for the main phytochemical classes, organoleptic physico-chemical parameters, extract mass, moisture content, pH, relative density, refractive index, phenolic and total flavonoid contents, color density, Hue tint, and tests biological factors for protection factor, DPPH free radical reduction activities, antifungal and antibacterial. Phytochemistry has demonstrated the presence of several groups of important metabolites mainly in pharmaceutical use, visual color of the citrus yellow floral extract, aromatic and homogeneous, extract mass = 12.57%, moisture content = 6.21%, pH = 5.85, refractive index = 1.4217 nD, relative density 0.9044 g mL-1 20 °C, total phenolics = 238.83 mg GAE 100 g-1 dried extract, total flavonoids = 17.93 mg QC 100 g-1 dried extract, color density = 1.05, Hue color = 8.24, protection factor UVA and UVC, antioxidant activity with CI50 = 9.44 µg mL-1. Antifungal activity demonstrated inhibition zone only for Candida tropicalis between 10-6 mm and for Candida krusei between 18-5 mm, and antibacterial for Escherichia coli between 13-7 mm, Pseudomonas aeruginosa between 21-5 mm and Enterococcus faecalis between 32-17 mm. The hydroethanolic floral extract of Schubertia grandiflora demonstrated potential biological activities, characterizing this natural product for the development of pharmaceutical, biological and agricultural formulations.
References
Agra, M. F., Freitas, P. F. & Barbosa-Filho, J. M. (2007). Synopsis of the plants known as medicinal and poisonous in Northeast of Brazil. Revista Brasileira de Farmacognosia, 17(1), 114-140. https://doi.org/10.1590/S0102-695X2007000100021 DOI: https://doi.org/10.1590/S0102-695X2007000100021
Ajuru, M. G., Williams, L. F. & Ajuru, G. (2017). Qualitative and quantitative phytochemical screening of some plants used in ethnomedicine in the Niger Delta region of Nigeria. Journal of Food and Nutrition Sciences, 5(5), 198-205. https://doi.org/10.11648/j.jfns.20170505.16 DOI: https://doi.org/10.11648/j.jfns.20170505.16
Almeida, L. F. D., Paula, J. F., Almeida-Marques, R. V. D. & Hebling, J. (2017). Atividade inibitória de óleos essenciais vegetais frente à Candida glabrata, resistente a fluconazol. Revista Brasileira de Ciências da Saúde, 21(2), 133-138. https://doi.org/10.22478/ufpb.2317-6032.2017v21n2.24660 DOI: https://doi.org/10.22478/ufpb.2317-6032.2017v21n2.24660
Arambewela, L. S. & Ranatunge, T. (1991). Indole alkaloids from Tabernaemontana divaricata. Phytochemistry, 30(5), 1740-1741. https://doi.org/10.1016/0031-9422(91)84254-P DOI: https://doi.org/10.1016/0031-9422(91)84254-P
Arendrup, M. C. & Patterson, T. F. (2017). Multidrug-resistant Candida: epidemiology, molecular mechanisms, and treatment. The Journal of Infectious Diseases, 216(3), S445-S451. https://doi.org/10.1093/infdis/jix131 DOI: https://doi.org/10.1093/infdis/jix131
Ávila-Reyes, J. A., Almaraz-Abarca, N., Chaidez-Ayala, A. I., Ramírez-Noya, D., Delgado-Alvarado, E. A., Torres-Ricario, R., Naranjo-Jiménez, N. & Alanís-Bañuuelos, R. E. (2018). Foliar phenolic compounds of tem wild species of Verbenacea as antioxidants and specific chemomarkers. Brazilian Journal of Biology, 78(1), 98-107. https://doi.org/10.1590/1519-6984.07516 DOI: https://doi.org/10.1590/1519-6984.07516
Baptista, K. C. C., Nascimento, K. F., Souza, S. J. P., Burci, L. M. & SILVA, F. B. (2020). Infecções hospitalares por Candida sp. em pacientes internados em UTI. Revista Gestão & Saúde, 22(2), 66-81. https://doi.org/10.17648/1984-8153-rgs-v2n22-6 DOI: https://doi.org/10.17648/1984-8153-rgs-v2n22-6
Barbosa, W. L. R., Quiganard, E., Tavares, I. C. C., Pinto, L. N., Oliveira, F. Q. & Oliveira, R. M. (2004). Manual para análise fitoquímica e cromatográfica de extratos vegetais. Revista Cientifica da UFPA, 4, 1-19.
Bhadane, B. S., Patil, M. P., Maheshwari, V. L. & Patil, R. H. (2018). Ethnopharmacology, phytochemistry, and Biotechnological advances of family Apocynaceae: A review. Phytotherapy Research, 32(7), 1181-1210. https://doi.org/10.1002/ptr.6066 DOI: https://doi.org/10.1002/ptr.6066
Bobin, M. F., Raymond, M. & Martini, M. C. (1994). UVA/UVB absorption properties of natural products. Cosmetics and Toiletries, 109, 63-78.
Borges, L. P. & Amorim, V. A. (2020). Metabólitos secundários de plantas. Revista de Agrotecnologia, 11(1), 54-67.
Carvalho, G. G., Peres, G. C., Mendonça, R. M. C. & Santos Filho, E. X. (2020). Phytochemical prospection and antibacterial activity of native plants from the errado of goiás, Brazil. Journal of Pharmacognosy and Phytochemistry, 9(2), 29-37.
Casley-Smith, J. R. (1993). “Treatment of lymphedema of the arms and legs with 5,6-benzo-(alpha)-pyrone. The New England Journal of Medicine, 329(16), 1158-63. https://doi.org/10.1056/NEJM199310143291604 DOI: https://doi.org/10.1056/NEJM199310143291604
Camacho-Campos, C., Pérez-Hernández, Y., Valvidia-Ávila, A. & Rubio-Fontanills, Y. (2020). Evaluación fitoquímica, antibacteriana y molusquicida de extractos de hojas de Agave spp. Revista Cubana de Química, 32(3), 390-405.
Cavalcanti, Y. W., Almeida, L. F. D. & Padilha, W. W. N. (2011). Screening da atividade antifúngica de óleos essenciais sobre cepas de Candida. Odontologia Clínico-Científica, 10(3), 243-246.
Chang, C. C., Yang, M. H., Wen, H. M. & Chern, J. C. (2002). Estimation of total flavonoids content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis, 10(3), 178-182. https://doi.org/10.38212/2224-6614.2748 DOI: https://doi.org/10.38212/2224-6614.2748
Descole, H. R. & Meyer, T. (1941). Uma nueva Asclepidacea Argentina: Schubertia schreiteri. Instituto de Botánica Darwinion, 53-56.
Dhivya, R. & Manimegalai, K. (2013). Preliminary phytochemical screening and GC-MS profiling of ethanolic flower extract of Calotropis gigantea Linn. (Apocynaceae). Journal of Pharmacognosy and Phytochemistry, 2(3), 28-32.
Di Mambro, V. M. & Fonseca, M. J. V. (2006). Avaliação da eficácia fotoprotetora in vivo de formulações contendo extratos de Ginkgo biloba e Glycirrhiza glabra. In: XX Congresso Brasileiro de Cosmetologia. São Paulo, Brasil.
Duarte, J. L., Mota, L. J. T. & Almeida, S. S. M. S. (2014). Análise fitoquímica das folhas de Tabebuia serratifolia (Vahl.) Nicholson (Ipê Amarelo). Estação Científica, 4(1), 33-43.
Endress, M. E., Liede-Schumann, S. & Meve, U. (2014). An updated classification for Apocynaceae. Phytotaxa, 159(3), 175-194. http://dx.doi.org/10.11646/phytotaxa.159.3.2 DOI: https://doi.org/10.11646/phytotaxa.159.3.2
Ekalu, A., Ayo, R.G. O., James, H. D. & Hamisu, I. (2019). A mini-review on the phytochemistry and biological activities of selected Apocynaceae plants. Preprints. http://dx.doi.org/10.20944/preprints201906.0219.v1 DOI: https://doi.org/10.20944/preprints201906.0219.v1
Ferreira, I. C. F. R. & Abreu, R. M. V. (2007). Stress oxidativo, antioxidantes e fitoquímicos. Bioanálise, 4(2), 32-39. DOI: https://doi.org/10.5628/rpcd.07.02.257
Harborne, J. B. & Williams, C. A. (2000). Advances in flavonoid research since 1992. Phytochemistry, 55, 481-504. https://doi.org/10.1016/S0031-9422(00)00235-1 DOI: https://doi.org/10.1016/S0031-9422(00)00235-1
Joselin, J., Brintha, T. S. S., Florence, A. R. & Jeeva, S. (2012). Screening of select ornamental flowers of the family Apocynaceae for phytochemical constituents. Asian Pacific Journal of Tropical Disease, 2(1), S260-S264. https://doi.org/10.1016/S2222-1808(12)60162-5 DOI: https://doi.org/10.1016/S2222-1808(12)60162-5
Kittakoop, P., Mahidol, C. & Ruchirawat, S. (2014). Alkaloids as important Scaffolds in therapeutic drugs for the treatments of cancer, tuberculosis and smoking cessation. Current Topics in Medicinal Chemistry, 14(2), 239-252. DOI: https://doi.org/10.2174/1568026613666131216105049
Kordalewska, M. & Perlin, D. S. (2019). Identification of drug resistant Candida auris. Frontiers in Microbiology, 10, 1-13. https://doi.org/10.3389/fmicb.2019.01918 DOI: https://doi.org/10.3389/fmicb.2019.01918
Lombardi, J. A., Salino, A. & Temoni, L. G. (2005). Diversidade florística de plantas vasculares no município de Januária, Minas Gerais, Brasil. Lundiana, 6(1), 3-20.
María, R., Shirley, M., Xavier, C., Jaime, S., David, V., Rosa, S. & JODIE, D. 2018. Preliminary phytochemical screening, total phenolic content and antibacterial activity of thirteen native species from Guayas province Ecuador. Journal of King Sud University – Science, vol. 30, no. 4, pp. 500-505. https://doi.org/10.1016/j.jksus.2017.03.009 DOI: https://doi.org/10.1016/j.jksus.2017.03.009
Martucci, M. C., Salatino, A., Oliveira, L. F. A. M. & Gonçalves, C. P. (2021). Metodologias acessíveis para quantificação de flavonoides e fenóis totais em própolis. Revista Virtual Química, 13(1), 1-13. http://dx.doi.org/10.21577/1984-6835.20200131 DOI: https://doi.org/10.21577/1984-6835.20200131
Martínez, M., Mantilla, L. E., Toro, D. R. & García, J. H. G. (2016). Perfil químico y actividad antibacterial de los extractos de Peltigera laciniata (Merril ex Riddle) Gyeln. Revista Cubana de Plantas Medicinales, 21.
Matthaus, B. (2002). Antioxidant activity of extracts obtained from residues of different Oilseeds. Journal of Agricultural and Food Chemistry, 50, 3444-3452. https://doi.org/10.1021/jf011440s DOI: https://doi.org/10.1021/jf011440s
Menezes Filho, A. C. P., Santos, M. C. & Castro, C. F. S. (2020). Estudo fitoquímico, bioativo, fotoprotetor e físico-químico do extrato floral de algodãozinho do Cerrado [Cochlospermum regium Schrank. (Pilg.)] – Bixaceae. Arquivos Científicos, 3(2), 59-71. https://doi.org/10.5935/2595-4407/rac.immes.v3n2p59-71 DOI: https://doi.org/10.5935/2595-4407/rac.immes.v3n2p59-71
Mezza, G.N., Borgarello, A. V., Grosso, N. R., Fernandez, H., Pramparo, M. C. & Gayol, M. F. (2018). Antioxidant activity of rosemary essential oil fractions obtained by molecular distillation and their effect on oxidative stability of sunflower oil. Food Chemistry, 242, 9-15. https://doi.org/10.1016/j.foodchem.2017.09.042 DOI: https://doi.org/10.1016/j.foodchem.2017.09.042
Nagadesi, P. K. & Kannamba, B. (2019). Effect of extraction and solvent on mycochemicals and proximate composition of lignicolous fungi Ganoderma lucidum and G. applanatum: A comparative study. SCIREA Journal of Pharmacy, 3(1), 1-24.
Nascimento, E. M. M., Rodrigues, F. F. G., Campos, A. R. & Costa, J. G. M. (2009). Phytochemical prospection, toxicity and antimicrobial activity of Mentha arvensis (Labiatae) from Northeast of Brazil. Journal of Young Pharmacists, 1(3), 210-212. https://doi.org/10.4103/0975-1483.57066 DOI: https://doi.org/10.4103/0975-1483.57066
Oladeji, O. S., Odelade, K. A. & Oloke, J. K. (2020). Phytochemical screening and antimicrobial investigation of Moringa oleifera leaf extracts. African Journal of Science, Technology, Innovation and Development, 12(1), 79-84. https://doi.org/10.1080/20421338.2019.1589082 DOI: https://doi.org/10.1080/20421338.2019.1589082
O’neil, L., Kim-Standage, W. S., Hughes, G. B. & Murray, K. B. (2000). In: Watson, R. R., 2000, Ed. Vegetables, Fruits and Herbs in Health Promotion, C. R. C. Press, USA.
Orak, H. H.; Bahrisefit, I. S. & Sabudak, T. (2019). Antioxidant activity of extracts of soursop (Annona muricata L.) leaves, fruit pulps, peels, and seeds. Polish Journal of Food and Nutrition Sciences, 69(4), 359-366. http://dx.doi.org/10.31883/pjfns/112654 DOI: https://doi.org/10.31883/pjfns/112654
Owen, L., White, A. W. & Laird, K. (2018). Characterisation and screening of antimicrobial essential oil components against clinically important antibiotic-resistant bacteria using thin layer chromatography-direct bioautography hyphenated with GC-MS, LC-MS and NMR. Phytochemical Analysis, 30(2), 1-11. https://doi.org/10.1002/pca.2797 DOI: https://doi.org/10.1002/pca.2797
Pandey, A. & Tripathi, S. (2014). Concept of standardization, extration and pre phytochemical screening strategies for herbal drug. Journal of Pharmacognosy and Phytochemistry, 2(5), 115-119.
Pattnaik, P. K., Kar, D., Chhatoi, H., Shahbazi, S., Ghosh, G. & Kuanar, A. (2017). Chemometric profile & antimicrobial activities of leaf extract of Calotropis procera and Calotropis gigantea. Natural Product Research, 31(16), 1954-1957. https://doi.org/10.1080/14786419.2016.1266349 DOI: https://doi.org/10.1080/14786419.2016.1266349
Pereira, M. M., Jácome, R. L. R. P., Alcântara, A. F. C., Alves, R. B. & Raslan, D. S. (2007). Alcalóides indólicos isolados de espécies do gênero Aspidosperma (Apocynaceae). Química Nova, 30(4), 970-983. https://doi.org/10.1590/S0100-40422007000400037 DOI: https://doi.org/10.1590/S0100-40422007000400037
Rapini, A. 2012. Taxonomy “under construction”: advances in the systematics of Apocynaceae, with emphasis on the Brazilian Asclepiadoideae. Rodriguésia, vol. 63, no. 1, pp. 075-088. https://doi.org/10.1590/S0100-40422007000400037 DOI: https://doi.org/10.1590/S2175-78602012000100007
Řezníčková, E., Guckýa, T., Kováčováa, V., Ajanib, H., Jordaa, R. & Kryštofa, V. (2019). Activity of 2,6,9-trisubstituted purines as potente PDGFRα kinase inhibitors with antileukemic activity. European Journal of Medicinal Chemistry, 182, 111663. https://doi.org/10.1016/j.ejmech.2019.111663 DOI: https://doi.org/10.1016/j.ejmech.2019.111663
Ribeiro, D.A., Camilo, C. J., Nonato, C. F. A., Rodrigues, F. F. G. and Menezes, I. A. (2020). Influence of seasonal variation on phenolic content and in vitro antioxidant activity of Secondatia floribunda A. DC. (Apocynaceae). Food Chemistry, 315, 126277. https://doi.org/10.1016/j.ejmech.2019.111663 DOI: https://doi.org/10.1016/j.foodchem.2020.126277
Santos, E. P., Freitas, Z. M., Souza, K. R., Garcia, S. & Vergnanini, A. (1999). In vitro and in vivo determinations of sun protection factors of sunscreen lotions with octylmethoxycinnamate. International Journal of Cosmetic Science, 21, 1-5. https://doi.org/10.1046/j.1467-2494.1999.181658.x DOI: https://doi.org/10.1046/j.1467-2494.1999.181658.x
Santos-Filho, F. S., Almeida Jr, E. B., Lima, P. B. & Soares, C. J. R. S. (2015). Checklist of the floral of the restingas of Piauí state, Northeast Brazil. Check List, 11(2), 1-10. http://dx.doi.org/10.15560/11.2.1598 DOI: https://doi.org/10.15560/11.2.1598
Schessl, M. (1999). Floristic composition and structure of floodplain vegetation in the Northern Pantanal of Mato Grosso, Brazil. Phyton, 39(2), 303-336.
Sethi, A., Prakash, R., Bhatia, A., Bhatia, G., Khanna, A. K. & Srivastava, S. P. (2012). Hypolipidemic, hypoglycemic and antioxidant activities of flower extracts of Allamanda violacea A.DC. (Apocynaceae). Tropical Journal of Pharmaceutical Research, 11(2), 225-234. https://doi.org/10.4314/tjpr.v11i2.8 DOI: https://doi.org/10.4314/tjpr.v11i2.8
Skenderidis, P., Kerasioti, E., Karkanta, E., Stagos, D., Kouretas, D., Petrotos, K., Hadjichristodoulou, C. & Tsakalof, A. (2018). Assessment of the antioxidant and antimutagenic activity of extracts from goji berry of greek cultivation. Toxicology Reports, 5, 251-257. https://doi.org/10.1016/j.toxrep.2018.02.001 DOI: https://doi.org/10.1016/j.toxrep.2018.02.001
Souza, T. M., Moreira, R. R. D., Rangel, V. L. B. I. & Pietro, R. C. L. R. (2005). Avaliação da atividade fotoprotetora de Achillea millefolium L. Revista Brasileira de Farmacognosia, 15, 36-38. https://doi.org/10.1590/S0102-695X2005000100008 DOI: https://doi.org/10.1590/S0102-695X2005000100008
Sonam, M., Singh, R. P. & Pooja, S. (2017). Phytochemical screening and TLC profiling of various extracts of Reinwardtia indica. International Journal of Pharmacognosy and Phytochemical Research, 9(4), 523-527. https://doi.org/10.1590/S0102-695X2005000100008 DOI: https://doi.org/10.25258/phyto.v9i4.8125
Thankamani, V. I., James, J., Veetil, A. K. T. & Sagadevan, L. D. M. (2011). Phyto chemical screening and anti microbial activity of Alstonia scholaris flowers (L) R. BR. Fam: Apocynaceae. International Journal of Pharmaceutical Research and Development, 3(3), 172-178.
Violante, I. M. P., Souza, I. M., Venturini, C. L., Ramalho, A. F. S., Santos, R. A. N. & Ferrari, M. (2009). Avaliação in vitro da atividade fotoprotetora de extratos vegetais do cerrado de Mato Grosso. Revista Brasileira de Farmacognosia, 19(2A), 452-457. http://dx.doi.org/10.1590/S0102-695X2009000300020 DOI: https://doi.org/10.1590/S0102-695X2009000300020
Wen, S., Chen, Y., Lu, Y., Wang, Y., Ding, L. & Jiang, M. (2016). Cardenolides from the Apocynaceae family and their anticancer activity. Fitoterapia, 112, 74-84. https://doi.org/10.1016/j.fitote.2016.04.023 DOI: https://doi.org/10.1016/j.fitote.2016.04.023
Wong, S. K., Lim, Y. Y. & Chan, E. W. C. (2013). Botany, uses, phytochemistry and pharmacology of selected Apocynaceae species: A review. Pharmacognosy Communications, 3(3), 2-11.
Wong, S. K., Lim, Y. Y., Abdullah, N. R. & NORDIN, F. J. (2011). Antiproliferative and phytochemical analyses of leaf extracts of tem Apocynaceae species. Pharmacognosy Research, 3(2), 100-106. https://dx.doi.org/10.4103%2F0974-8490.81957 DOI: https://doi.org/10.4103/0974-8490.81957
Yadav, R., Khare, R. K. & Singhal, A. (2017). Qualitative phytochemical screening of some selected medicinal plants of Shivpuri disctrict (M.P.). International Journal Life-Sciences Scientific Research, 3(1), 844-847. https://doi.org/10.21276/IJLSSR.2017.3.1.16 DOI: https://doi.org/10.21276/ijlssr.2017.3.1.16
Yadang, S. A. F., Sotoing, G.T., Zouakeu, K. S. N. & Khan, M. A. (2019). Quantification of bioactive compounds and evaluation of the antioxidant activity of Carissa edulis Vahl (Apocynaceae) leaves. The Scientific World Journal, 2019, 1-9. DOI: https://doi.org/10.1155/2019/7549620
Yalavarthi, C. & Thiruvengadarajan, V. S. (2013). A review on identification strategy of phyto constituents present in herbal plants. International Journal of Research in Pharmaceutical Sciences, 4(2), 123-140.
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Copyright (c) 2022 Antonio Carlos Pereira de Menezes Filho, Matheus Vinicius Abadia Ventura, Carlos Frederico de Souza Castro, Rogério Favareto, Celso Martins Belisário, Marconi Batista Teixeira, Frederico Antônio Loureiro Soares
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