Active gelatin-based films incorporated with juçara (Euterpe edulis M.) extract: Mechanical, thermal, and antioxidant properties
DOI:
https://doi.org/10.14295/bjs.v5i5.852Keywords:
juçara extract, biodegradable packaging, bioactive properties, antioxidant activity, Euterpe genusAbstract
The replacement of conventional plastic packaging with biodegradable materials derived from natural sources has driven research toward functional biopolymeric systems. Gelatin is a promising matrix for film formation, and juçara (Euterpe edulis) extract, rich in phenolic compounds, represents a potential bioactive additive. This study aimed to develop gelatin-based films incorporating juçara extract at concentrations of 0.1 g extract g⁻¹ gelatin (S1) and 0.25 g extract g⁻¹ gelatin (S2), in comparison with a control film without extract. The films were characterized in terms of water solubility, mechanical properties, water vapor permeability (WVP), color attributes, morphology, thermal behavior, and antioxidant activity. Data were statistically analyzed by analysis of variance followed by Tukey’s test (p ≤ 0.05). Extract incorporation significantly affected film properties. S2 exhibited the highest elongation at break (24.24%) and the lowest WVP (1.57 g mm m⁻² day⁻¹ kPa⁻¹), although tensile strength decreased compared to the control (22.81 MPa). Films containing juçara extract showed reduced solubility relative to the control. Colorimetric analysis indicated similarity to fresh fruit, with increased opacity upon extract addition. Scanning electron microscopy revealed uniform and homogeneous surfaces, with greater particle dispersion observed in S2. Thermal analysis demonstrated a glass transition temperature of 36.9 °C for all samples, while melting temperatures increased with extract concentration, reaching 142.3 °C in S2. Antioxidant activity increased markedly after extract incorporation, with inhibition percentages of 1.97%, 38.68%, and 55.91% for control, S1, and S2, respectively. The results indicate that Euterpe edulis extract enhances both functional and bioactive properties of gelatin-based films, particularly at 0.25 g extract g⁻¹ gelatin, supporting its potential application in active biodegradable food packaging.
References
ASTM. (2000a) Standard test methods for tensile properties on thin plastic sheeting. In: Standard designation D882-02, Annual book of ASTM, pp. 162-170.
ASTM. (2000b) Standard test methods of water vapor transmission of materials. In: Standard designation E96/E96M-05, Annual book of ASTM, pp. 406-413.
Bandeira, S. F., & Pinto, L. A. A. (2015). Modified Gelatin Films from Croaker Skins: Effects of pH, and Addition of Glycerol and Chitosan. Journal of Food Process Engineering, 38(6), 613-620. https://doi.org/10.1111/jfpe.12191 DOI: https://doi.org/10.1111/jfpe.12191
Bastos, B. M., Farias, B. S., Casati, M. O., Engelmann, J. I., Moura, J. M., & Pinto, L. A. A. (2021). Gelatin Films from Carp Skin Crosslinked by Gallic Acid and Incorporated with Chitosan/Tuna Lipid Fractions. Journal of Polymers and the Environment, 29, 2096-2110. https://doi.org/10.1007/s10924-020-01995-2 DOI: https://doi.org/10.1007/s10924-020-01995-2
Bhattarai, S., & Janaswamy, S. (2023). Biodegradable, UV-blocking, & antioxidant films from lignocellulosic fibers of spent coffee grounds. International Journal of Biological Macromolecules, 253(P2), 126798. https://doi.org/10.1016/j.ijbiomac.2023.126798 DOI: https://doi.org/10.1016/j.ijbiomac.2023.126798
Borges, G. S., C., Vieira, G. K. F., Copetti, C., Gonzaga,V. L., Zambiazi, R. C., Filho, M. J., & Fett, R. (2011). Chemical characterization, bioactive compounds, and antioxidant capacity of jussara (Euterpe edulis) fruit from the Atlantic Forest in southern Brazil. Food Research International, 44(7), 2128-2133. https://doi.org/10.1016/j.foodres.2010.12.006 DOI: https://doi.org/10.1016/j.foodres.2010.12.006
Choi, J., Lee, J.-S., Han, J., & Chang, Y. (2023). Development of gelatin – sodium caseinate high-oxygen-barrier film containing elderberry (Sambucus nigra L .) extract and its antioxidant capacity on pork. Food Bioscience, 53, 102617. https://doi.org/10.1016/j.fbio.2023.102617 DOI: https://doi.org/10.1016/j.fbio.2023.102617
Crozatti, T. T. S., Mangolim, C. S., Larentins, P. V., De Mello, J. C. P., & Matioli, C. (2023). Extraction, microencapsulation, and application of anthocyanins from juçara palm fruit (Euterpe edulis Mart.): enhancement of natural pigment. Journal of Food Science and Tecnology, 60(1), 361-371. https://doi.org/10.1007/s13197-022-05623-w DOI: https://doi.org/10.1007/s13197-022-05623-w
Ding, F., & Fu, L. (2025). Recent advances in gelatin-cellulose composite films for food packaging applications. International Journal of Biological Macromolecules, 321(P1), 146135. https://doi.org/10.1016/j.ijbiomac.2025.146135 DOI: https://doi.org/10.1016/j.ijbiomac.2025.146135
Fakhouri, F. M., Fontes, L. C. B., Gonçalves, P. V. de M., Milanez, C. R., Steel, C. J., & Collares-Queiroz F. P. (2007). Films and edible coatings based on native starches and gelatin in the conservation and sensory acceptance of Crimson grapes. Food Science and Technology, 27(2), 369–375. https://doi.org/10.1590/S0101-20612007000200027 DOI: https://doi.org/10.1590/S0101-20612007000200027
Farokhi, N. M., Milani, J. M., & Amiri, Z. R. (2024). Fabrication of nanocomposite gelatin-based film by the pickering emulsion containing nanoparticles of chitin. Journal of Food Engineering, 367(8), 111885. http://doi.or/10.1016/j.jfoodeng.2023.111885 DOI: https://doi.org/10.1016/j.jfoodeng.2023.111885
Filho, J. G. O., Bertolo, M. R. V., Fernandes, S. S., Lemes, A. C., Silva, G. C., Junior, S. B., Zeredo, H. M. C., Mattoso, L. H. C., & Egea, M. B. (2024). Intelligent and active biodegradable biopolymeric films containing carotenoids. Food Chemistry. 434, 137454. https://doi.org/10.1016/j.foodchem.2023.137454 DOI: https://doi.org/10.1016/j.foodchem.2023.137454
He, H., Wang, Y., Gao, J., Huang, Z., Liu, Y., Tian, G., Xu, L., & Zhu, Z. (2022). Recycled polyethylene/polyethylene-ethylene-1-octene-maleic anhydride composite with improved mechanical properties. Journal of Applied Polymer Science, 139(8), 51694. https://doi.org/10.1002/app.51694 DOI: https://doi.org/10.1002/app.51694
Jamróz, E., Tkaczewska, J., Juszczak, L., Zimowska, M., Kawecka, A., Krzysciak, P., & Skóra, M. (2022). The influence of lingonberry extract on the properties of novel, double-layered biopolymer films based on furcellaran, CMC and a gelatin hydrolysate. Food Hydrocolloids, 124(21), 107334. https://doi.org/10.1016/j.foodhyd.2021.107334 DOI: https://doi.org/10.1016/j.foodhyd.2021.107334
Kadam, S. U., Pankaj, S. K., Tiwari, B. K., Cullen, P. J., & O’Donnell, C. P. (2015). Development of biopolymer-based gelatin and casein films incorporating brown seaweed Ascophyllum nodosum extract. Food Packaging and Shelf Life, 6, 68-74. http://doi.org/10.1016/j.fpsl.2015.09.003 DOI: https://doi.org/10.1016/j.fpsl.2015.09.003
Khedri, S., Sadeghi, E., Rouhi, M., Delshadian, Z., Mortazavian, A. M., Guimarães, J. T., Fallah, M., & Mohammadi, R. (2021). Bioactive edible films: Development and characterization of gelatin edible films incorporated with casein phosphopeptides. Food Science and Technology - LWT, 138(4), 110649. http://doi.org/10.1016/j.lwt.2020.110649 DOI: https://doi.org/10.1016/j.lwt.2020.110649
Kola, V., & Carvalho, I. S. (2023). Plant extracts as additives in biodegradable films and coatings in active food packaging. Food Bioscience, 54, 102860. https://doi.org/10.1016/j.fbio.2023.102860 DOI: https://doi.org/10.1016/j.fbio.2023.102860
Lu, Y., Luo, Q., Chu, Y., Tao, N., Deng, S., Wang, L., & Li, L. (2022). Application of gelatin in food packaging: a review. Polymers, 14(3), 436. https://doi.org/10.3390/polym14030436 DOI: https://doi.org/10.3390/polym14030436
Mohan, S., Unnikrishnan, T. G., Dubey, U., Ramesh, M., & Panneerselvam, K. (2023). Development and characterization of mustard oil incorporated biodegradable chitosan films for active food packaging applications. Journal of Polymers and the Environment, 31, 2190-2203. http://doi.org/10.1007/s10924-022-02719-4 DOI: https://doi.org/10.1007/s10924-022-02719-4
Mondal, K., Bhattacharjee, S. K., Mudenur, C., Ghosh, T., Goud, V. V., & Katiyar, V. (2022). Development of antioxidant-rich edible active films and coatings incorporated with de-oiled ethanolic green algae extract: a candidate for prolonging the shelf life of fresh produce. Croyal Society of Chemistry, 12, 13295-13313. http://doi.org/10.1039/d2ra00949h DOI: https://doi.org/10.1039/D2RA00949H
Nakashima, A. Y., Chevalier, R. C., & Cortez-Vega, W. R. (2016). Development and characterization of collagen films with added essential oil of clove india. Journal of Bioenergy and Food Science, 3(1), 50-57. https://doi.org/10.18067/jbfs.v3i1.86 DOI: https://doi.org/10.18067/jbfs.v3i1.86
Peighambardoust, S. H., Fasihnia, S. H., Peighambardoust, S. J., Pateiro, M., Domínguez, R. & Lorenzo, J. M. (2021). Active polypropylene-based films incorporating combined antioxidants and antimicrobials: preparation and characterization. Foods, 10(4), 722. https://doi.org/10.3390/foods10040722 DOI: https://doi.org/10.3390/foods10040722
Pessanha, K. L. F., Farias, M. G., Carvalho, C. W. P., & Godoy, R. L. de O. (2018). Starch Films Added of Açaí Pulp (Euterpe oleracea Martius). Brazilian Archives of Biology Technology, 61, e181700824. https://doi.org/10.1590/1678-4324-2018170824 DOI: https://doi.org/10.1590/1678-4324-2018170824
Rangaraj, V. M., Rambabu, K., Banat, F., & Mittal, V. (2021). Effect of date fruit waste extract as an antioxidant additive on the properties of active gelatin films. Food Chemistry, 355(2), 129631. http://doi.org/10.1016/j.foodchem.2021.129631 DOI: https://doi.org/10.1016/j.foodchem.2021.129631
Said, N. S., & Sarbon, N. M. (2022). Physical and mechanical characteristics of gelatin-based films as a potential food packaging material: A review. Membranes, 12(5), 442. http://doi.org/10.3390/membranes12050442 DOI: https://doi.org/10.3390/membranes12050442
Satapathy, S., & Palanisamy, A. (2021). Mechanical and barrier properties of polyvinyl chloride plasticized with dioctyl phthalate, epoxidized soybean oil, and epoxidized cardanol. Journal of Vinyl & Additive Technology, 27(3), 599-611. https://doi.org/10.1002/vnl.21831 DOI: https://doi.org/10.1002/vnl.21831
Silva, I. C. F., Reis, M. H. M., Madrona, G. S., & Bergamasco, R. C. (2025). Stability of anthocyanins: investigating the impact of caffeic acid and rutin in microcapsules containing acidified extract from Juçara palm fruit. Food Chemistry, 496, 146751. https://doi.org/10.1016/j.foodchem.2025.146751 DOI: https://doi.org/10.1016/j.foodchem.2025.146751
Siripatrawan, U., & Harte, B. R. (2010). Physical properties and antioxidant activity of an active film from chitosan incorporated with green tea extract. Food Hydrocolloids, 24(8), 770-775. http://doi.org/10.1016/j.foodhyd.2010.04.003 DOI: https://doi.org/10.1016/j.foodhyd.2010.04.003
Takma, D. K., Bozkurt, S., Koç, M., Korel, F., & Nadeem, H. S. (2024). Optimizing a bionanocomposite film for active food packaging with pectin, gelatin, and chestnut shell extract-loaded zein nanoparticles. Food Packaging Shelf, 42, 101243. https://doi.org/10.2139/ssrn.4596166 DOI: https://doi.org/10.1016/j.fpsl.2024.101243
Villasante, J., Martin-Lujano, A., & Almajano, M. P. (2020). Characterization and application of gelatin films with pecan walnut and shell extract (Carya illinoiensis). Polymers, 12(6), 1424. http://doi.org/10.3390/polym12061424 DOI: https://doi.org/10.3390/polym12061424
Yong, H., Liu, J., Qin, Y., Bai, R., Zhang, X., & Liu, J. (2019). Antioxidant and pH-sensitive films developed by incorporating purple and black rice extracts into chitosan matrix. International Journal of Biological Macromolules, 137, 307-316. https://doi.org/10.1016/j.ijbiomac.2019.07.009 DOI: https://doi.org/10.1016/j.ijbiomac.2019.07.009
Zhang, Q., Zhang, J., Ping, Q., Sui, Z., & Li, H. (2026). Performance comparison of glycerol-plasticized type A and type B gelatin films and their suitability for food packaging. Polymer, 343, 129389. https://doi.org/10.1016/j.polymer.2025.129389 DOI: https://doi.org/10.1016/j.polymer.2025.129389
Zhang, Z., Jiang, P., Wai, P. T., Feng, S., Lu, M., Zhang, P., Leng, T., Pan, L., & Pan, J. (2022). Construction and synthesis of high-stability biobased oligomeric lactate plasticizer: applicable to PVC and PLA polymers. Industrial & Engineering Chemistry Research, 62(35), 12931-12941. https://doi.org/10.1021/acs.iecr.2c02137 DOI: https://doi.org/10.1021/acs.iecr.2c02137
Zighed, M., & Benotmane, B. (2022). Performance of high-density polyethylene-starch-linen fiber biocomposite. Iranian Polymer Journal, 31, 751-760. https://doi.org/10.1007/s13726-022-01035-x DOI: https://doi.org/10.1007/s13726-022-01035-x
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Joelmir Grassi Presente, Marta dos Santos Diniz Freitas, Bruna Moura Bastos, Neusa Fernandes de Moura, Roberto de Souza Gomes da Silva

This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
