Analysis of Kigelia africana (Lam.) Benth. fruit powder's antioxidant and phytochemical properties

Authors

DOI:

https://doi.org/10.14295/bjs.v3i7.596

Keywords:

Kigelia africana, food safety, phytochemical, antioxidants, metabolites

Abstract

The purpose of this study was to investigate the antioxidant and phytochemical properties of Kigelia africana fruit powder. Fresh K. africana fruit was air-dried and processed into powder. K. africana fruit powder contained saponins (35.86 mg/100 g-1), tannins (106.1 mg/100 g-1), phenols (1340.6 mg/100 g-1), flavonoids (985.11 mg/100 g-1), steroids (81.20 mg/100 g-1), glycosides (18.32 mg/100 g-1), triterpenes (90.65 mg/100 g-1), and alkaloids (51.22 mg/100 g-1). The study found that phenolic chemicals were the most concentrated, followed by flavonoids, tannins, triterpenes, steroids, alkaloids, saponins, and glycosides. The antioxidant activities of Kigella africana fruit meal revealed that it contains lipid peroxidation (71.80%), 2,2-diphenyl-1-picrylhydrazyl (DPPH) (56.02%), vitamin C (6.22%), 2,2’-Azino-bis(3-Ethylbenzothiazoline-6-Sulfonic acid) (ABTS) (26.11%), and hydroxyl radical inhibition (45.92%). Therapeutically, the phytochemicals revealed that K. africana fruit has antihelminthic, antidiarrheal, antimicrobial, antifungal, antimicrobial, immunomodulatory, hypoglycemic, antioxidant, hypocholesterolemic, hepatoprotective, hepatostimulatory, analgesic, and anticarcinogenic properties. The presence of lipid peroxidation, vitamin C, DPPH, and ABTS in Kigelia africana fruit indicates that it can be employed as an antioxidant supplement in animal diets.

References

Abbas, Z., Mustafa, S., Khan, M. F., Khan, M. A., Massey, S., Dev, K., Hkan, A., Parveen, S., & Husain, S. A. (2024). Therapeutic importance of Kigelia africana subsp. Africana: an alternative medicine. Natural Product Research, https://doi.org/10.1080/14786419.2023.2273914 DOI: https://doi.org/10.1080/14786419.2023.2273914

Atolani, O., Adeyemi, S. O., Akpan, E., Adeosun, C. B., & Olatunji, G. A. (2011). Chemical composition and antioxidant potentials of kigelia pinnata root oil and extracts. Excli Journal Experimental and Clinical Sciences, 10, 264-273. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5611606/

Abdulkadir, M. N., Adedokun, A., & John, E. (2015). Phytochemical composition and antimicrobial evaluation of Kigelia africana Lam. Asian Journal of Plant Science and Research, 5, 14-17. https://hal.science/hal-03691595/

Adewale, A. O., Alagbe, J. O., & Adeoye, A. O. (2021). Dietary supplementation of Rauvolfia vomitoria root extract as a phytogenic feed additive in growing rabbit diets: Haematology and serum biochemical indices. International Journal of Current Researches in Sciences, Social and Languages, 1(3), 15-26. https://www.ijcrsssl.com/papers/v1/i3/IJCRSSSLV1I30003.pdf

Alagbe, J. O., Shittu, M. D., & Ushie, F. T. (2021). GC-MS analysis of methanolic stem bark extract of Zollingeriana indigofera. Asian Journal of Advances in Research, 4(1), 1265-1267. http://archives.articleproms.com/id/eprint/2064

Alagbe, J. O (2021). Dietary Supplementation of Rauvolfia vomitoria root extract as a phytogenic feed additive in growing rabbit diets: Growth performance and caecal microbial population. Concept of Dairy & Veterinary Sciences, 4(2), 409-416. http://dx.doi.org/10.32474/CDVS.2021.04.000184

Alagbe, J. O. (2022). Characterization of bioactive compounds in Luffa aegyptiaca leaf ethanolic extracts using gas chromatography and mass spectrometry. Food Science & Applied Microbiology Reports, 1(2), 21-28. https://doi.org/10.61363/fsamr.v1i2.53

Alagbe, J. O., Shittu, M. D., & Tanimomo, B. K. (2022). Influence of Anogeissusleio carpus stem bark on the fatty acid composition in meat of broiler chickens. European Journal of Life Safety and Stability 14(22), 13-22. http://www.ejlss.indexedresearch.org/index.php/ejlss/article/view/447

Alagbe, J. O (2022). Use of medicinal plants as a panacea to poultry production and food security: A review. American Journal of Technology and Applied Sciences, 1, 24-36. https://www.americanjournal.org/index.php/ajtas/article/view/4

Alagbe, J. O., Muhammad, R. S., Daniel, S. M., & Christiana, O. O. (2022). Effect of Trichilia monadelpha stem bark extract on the fatty acid composition of rabbit’s thigh meat. Journal of Environmental Issues and Climate Change, 1(1), 63-71. https://doi.org/10.59110/jeicc.v1i1.76 DOI: https://doi.org/10.59110/jeicc.v1i1.76

Alagbe, J. O., Agubosi, O. C., & Oluwafemi, R. A. (2023). Histopathology of broiler chickens fed diets supplemented with Prosopis africana (African mesquite) essential oil. Brazilian Journal of Science, 2(9), 49-59. https://doi.org/10.14295/bjs.v2i9.385 DOI: https://doi.org/10.14295/bjs.v2i9.385

Alagbe, O. J. (2023). Sensory evaluation and fatty acid composition of broiler chickens fed diets containing Prosopis africana oil. Journal of Healthcare and Biomedical Science, 1(2), 36-45. https://doi.org/10.31098/jhbs.v1i2.1721 DOI: https://doi.org/10.31098/jhbs.v1i2.1596

Alagbe, O. J., Agubosi, O. C. P., Oluwafemi, O. L., Akande, T. O., Adegbite, A. E., & Emiola, I. A. (2023). Haemato-biochemical indices and intestinal microbial population of broiler chickens fed diet supplemented with Prosopis africana (African mesquite) essential oil. Brazilian Journal of Science, 2(9), 98-110. https://doi.org/10.14295/bjs.v2i9.390 DOI: https://doi.org/10.14295/bjs.v2i9.390

Alagbe, J. O., Kadiri, M. C., Oluwafemi, R. A., Agubosi, O. C. P., & Anorue, D. N. (2023). Analysis of bioactive compounds in ethanolic extracts of Xylopia aethiopica leaves using gas chromatography and mass spectrometry technique. American Journal of Science on Integration and Human Development, 1(1), 1-10. http://grnjournal.us/index.php/AJSIHD/article/view/4

Alagbe, J. O. (2023). Bioactive compounds in ethanolic extract of Strychnos innocua root using gas chromatography and mass spectrometry (GC-MS). Drug Discovery, 17, e4dd1005. https://doi.org/10.54905/disssi.v17i39.e4dd1005 DOI: https://doi.org/10.54905/disssi.v17i39.e4dd1005

Alagbe, J. O., Shittu, M. D., Adesina, A. Y., Grace, C. J., Cincinsoko, K. M., Oluwafemi, B. S., Erikanobong, E. (2024). The approximate mineral and phytochemical content of the leaves, stem bark, and roots of Pterocarpus erinaceus in India. Corrado: Agricultural and Biological Science, 1(1), 32-41. https://doi.org/10.14295/cerrado.v1i1.562 DOI: https://doi.org/10.14295/cerrado.v1i1.562

Benderitter, M., Maupoli, V., Vergely, C., Dalloz, F., Briot, F., & Rochette, L. (1998). Studies by electron paramagnetic resonance of the importance of iron in the hydroxyl scavenging properties of ascorbic acid in plasma: Effects of iron chelators. Fundamental & Clinical Pharmacology, 12(5), 510-516. https://doi.org/10.1111/j.1472-8206.1998.tb00979.x DOI: https://doi.org/10.1111/j.1472-8206.1998.tb00979.x

Biswas, A., Dey, S., Li, D., You, L., Zhang, J., & Huang, S. (2020). Comparison of phytochemical profile, mineral content, and in vitro antioxidant activities of Corchorus capsularis and Corchorus olitorius leaf extracts from different populations. Journal of Food Quality, 2931097. https://www.hindawi.com/journals/jfq/2020/2931097/ DOI: https://doi.org/10.1155/2020/2931097

Bodiwala, K. B., Banker, N., Lalwani, R., Shah, M. B., & Gajjar, A. K. (2024). Quality control methods for fruit extracts of Kigelia africana using high performance thin layer chromatography. Natural Product Research. https://doi.org/10.1080/14786419.2024.2340762 DOI: https://doi.org/10.1080/14786419.2024.2340762

Chandra, S., & Goyal, A. T. (2014). Antioxidant activity by DPPH radical scavenging method of Ageratum conyzoides Linn. Leaves. American Journal of Ethnomedicine, 1(4), 244-249.

Cowan, M. M. (1999). Plant products as antimicrobial agents. Clinical Microbiology. Reviews, 12, 564-582. https://doi.org/10.1128/cmr.12.4.564 DOI: https://doi.org/10.1128/CMR.12.4.564

Dudonnѐ, S., Vitrac, X., Coutiѐrep, P., Woillez, M., & Mѐrilon, J. (2009). Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD and ORAC assays. Journal of Agricultural and Food Chemistry, 57, 1768-1774. https://doi.org/10.1021/jf803011r DOI: https://doi.org/10.1021/jf803011r

Fagbohun, O. F., Babalola, O. O., Agboola, F. K., Joseph, J. S., Malindisa, S., & Msagati, T. A. M. (2020). Evaluation of phytochemicals, antioxidants, trace elements in Kigelia africana fruit extracts and chemical profiling analysis using UHPLC-qTOF-MS2 spectrometry. Biological Trace Element Research, 195, 679-695. https://doi.org/10.1007/s12011-019-01869-2 DOI: https://doi.org/10.1007/s12011-019-01869-2

Gupta, O. P., Sing, S., Bani, S., Sharma, N., Malhotra, S., Gupta, B. D., Banerjee, S. K., & Handa, S. S. (1999). Anti-inflammatory and anti-arthritic activities of silymarin acting through inhibition of 5-lipoxygenase. Phytomedicine, 7(1), 21-24. https://doi.org/10.1016/S0944-7113(00)80017-3 DOI: https://doi.org/10.1016/S0944-7113(00)80017-3

Halder, S., & Sharma, A. (2017) A review on Kigelia africana. World Journal of Pharmaceutical Research, 6(11), 389-411. DOI: https://doi.org/10.20959/wjpr201711-9633

Halliwell, B. (2007). Biochemistry of oxidative stress. Biochemical Society Transactions, 35(5), 1147-1150. DOI: https://doi.org/10.1042/BST0351147

Harris, J. C., Cottrell, S. L., Plummer, S., & Lloyd, D. (2001). Antimicrobial properties of Allium sativum (garlic). Applied Microbiology and Biotechnology, 57, 282-286. https://doi.org/10.1007/s002530100722 DOI: https://doi.org/10.1007/s002530100722

Khaled, M. A., Hossam, S. E., Heba, I. M., Tarek, A. S., Ahmed, G., Abdallah, T. M., Mohammed, A. A., & Falam, S. A. (2022). Antioxidant, anticancer activity and phytochemical profiling of Kigelia pinnata fruits. Separations, 9(11), 379-388. https://doi.org/10.3390/separations9110379 DOI: https://doi.org/10.3390/separations9110379

Koné, M. C., Kpan, B. W., Kandé, I., Kouakou, R. K., Koman, R. S., Konan, Y., & Kouakou, K. (2021). Effect of different parts of Kigelia africana fruit aqueous extracts on sperm parameters and testis. Advances in Reproductive Sciences, 9, 171-188. https://doi.org/10.4236/arsci.2021.93017 DOI: https://doi.org/10.4236/arsci.2021.93017

Kumar, T. S., Shalini, S., Roy, T. A., Prasanna, S., Balasundaraprabhu, R., Sundaram, S. (2024). Solvent selection for anthrocyanin dye extraction from Kigelia africana and Hibiscus sabdariffa for dye sensitized solar cells. Journal of Photochemistry and Photobiology, 20, 100233. https://doi.org/10.1016/j.jpap.2024.100233 DOI: https://doi.org/10.1016/j.jpap.2024.100233

Macrae, R., Robinson, R. K., & Sadler, M. J. (1993). Encyclopaedia of food science, food technology and nutrition. London: Academic Press.

Madhu, M., Sailaja, V., Satyadev, T. N. V. S. S., & Satyanarayana, M. V. (2016). Quantitative phytochemical analysis of selected medicinal plant species by using various organic solvents. Journal of Pharmacognosy and Phytochemical, 5(2), 25-29.

Makkar, H. P. S., Norsambuu, T., Lkhavatsere, S., & Becker, K. (2009). Plant secondary metabolites in some medical plants of Mongolia used for enhancing animal health and production. Tropicultura, 27(3), 159-167.

Mohammed, H. A., Alshalmani, S. K., & Abdellatif, A. G. (2013). Antioxidant and quantitative estimation of phenolic and flavonoids of three halophytic plants growing in Libya. Journal of Pharmacognosy and Phytochemistry, 2(3), 89-94.

Ogunlakin, A. D., & Sonibare, M. A. (2023). Ameliorative effect of Kigelia africana (Lam.) Benth. fruit methanol extract in letrozole-induced polycystic ovarian syndrome rat. Kuwait Journal of Science, 50(4), 622-626. https://doi.org/10.1016/j.kjs.2023.05.018 DOI: https://doi.org/10.1016/j.kjs.2023.05.018

Ojediran, T. K., Emiola, I. A., Durojave, V., & Alagbe, J. O. (2024). Proximate, vitamin and GC-MS profiling of Kigelia africana fruit powder. Corrado: Agricultural and Biological Science, 1(1), 13-20. https://doi.org/10.14295/cerrado.v1i1.556 DOI: https://doi.org/10.14295/cerrado.v1i1.556

Oloruntola, O. D., & Ayodele, S. O. (2022). Phytochemical, proximate and mineral composition, antioxidant and antidiabetic properties evaluation and comparison of mistletoe leaves from moringa and kolanut trees. Turkish Journal of Agriculture-Food Science and Technology, 10(8), 1524-1531. https://doi.org/10.24925/turjaf.v10i8.1524-1531.5134 DOI: https://doi.org/10.24925/turjaf.v10i8.1524-1531.5134

Oloruntola, O. D. (2021) Proximate, phytochemical, mineral composition and antioxidant activity of Anacardium occidentale L. leaf powder. Dysona Life Science, 2, 39-49. https://doi.org/10.30493/dls.2021.290718

Oloruntola, O. D., Ayodele, S. O., Adeyeye, S. A., Fasuhami, O. S., Osowe, C. O., & Ganiyu, T. (2022). Proximate composition, phytochemical profile, antioxidant, antidiabetic, and anti-inflammatory properties of Justica carnea leaf powder. Black Sea Journal of Agriculture, 5(4), 415-423. https://doi.org/10.47115/bsagriculture.1145262 DOI: https://doi.org/10.47115/bsagriculture.1145262

Orabi, M. A., Ibrahim, N., Gad, H. A., & Choucry, M. A. (2023). Comparative study of the antioxidant and cytotoxicity activities of organs of Kigelia africana and their topoisomerase I inhibitory activities. South African Journal of Botany, 162, 838-846. https://doi.org/10.1016/j.sajb.2023.10.001 DOI: https://doi.org/10.1016/j.sajb.2023.10.001

Owolabi, O. J., & Omogbai, E. K. I. (2007). Analgesic and anti-inflammatory activities of the ethanolic stem bark extract of Kigelia africana (Bignoniaceae). African Journal of Biotechnology, 6(5), 582-585.

Picerno, P., Autore, G., Marzocco, S., Meloni, M., Sanogo, R., & Aquino, R. P. (2005). Anti-inflammatory activity of verminoside from Kigelia africana and evaluation of cutaneous irritation in cell cultures and reconstituted human epidermis. Journal of Natural Products, 68(11), 1610-161. https://doi.org/10.1021/np058046z DOI: https://doi.org/10.1021/np058046z

Rauha, J. P., Remes, S., Heinonen, M., Hopia, A., Kahkonen, M., Kujala, T., Pihlaja, K., Vuorela, H., & Vuorela, P. (2000). Antimicrobial effects of Finnish plant extracts containing flavonoids and other phenolic compounds. International Journal of Food Microbiology, 56(1), 3-12. https://doi.org/10.1016/S0168-1605(00)00218-X DOI: https://doi.org/10.1016/S0168-1605(00)00218-X

Sharma, S., John, A. O., Xing, L., Ram, S., & Amita, K. (2022). Comparative analysis of ethanolic Juniperus thurifera leaf, stem bark and root extract using gas chromatography and mass spectrometry. International Journal of Agriculture and Animal Production, 2(6), 18-27. https://doi.org/10.55529/ijaap.26.18.27 DOI: https://doi.org/10.55529/ijaap.26.18.27

Shittu, M. D., Alagbe, J. O., Ojebiyi, O. O., Ojediran, T. K., & Rafiu, T. A. (2022). Growth performance and haematological and serum biochemical parameters of broiler chickens given varied concentrations of Polyalthia longifolia leaf extract in place of conventional antibiotics. Animal Science and Genetics, 18(2), 57-71. DOI: https://doi.org/10.5604/01.3001.0015.9185

Talari, S., Rudroju, S., Penchala, S., & Nanna Rama, S. (2012) Quantification of total phenolics and total flavonoid contents in extracts of Oroxylum indicum I. Kurz. Asain Journal of Pharmaceutical and Clinical Research, 5(4), 177-179.

Turkoglu, S., Celik, S., Turkoglu, I., Cakilcioglu, U., & Bahsi, M. (2010). Determination of the antioxidant properties of ethanol and water extracts from different parts of Teucrium parviflorum Schreber. African Journal of Biotechnology, 9(40), 6797-6805. https://www.ajol.info/index.php/ajb/article/view/92546

Vera, B. (2021). Essential oils knowledge: From facts to farm or from farm to facts. International Poultry Magazine, 3(3), 2-4.

Weiss, C. R., Moideen, S. V., Croft, S. L., & Houghton, P. J. (2000) Activity of extracts and isolated naphthoquinones from Kigelia pinnata against Plasmodium falciparum. Journal of Natural Products, 63(9), 1306-1309. https://doi.org/10.1021/np000029g DOI: https://doi.org/10.1021/np000029g

Wilasrusmee, C., Kittur, S., Shah, G., Siddiqui, J., Bruch, D., Wilasrumee, S., & Kittur, D. S. (2002). Immunostimulatory effect of Silybum marianum (milk thistle) extract. Medical Science Monitor, 8, BR439-BR443. https://europepmc.org/article/med/12444368

Wills, R. B. H., Bone, K., & Morgan, M. (2000). Herbal products: active constituents, modes of action and quality control. Nutrition Research Reviews, 13(1), 47-77. https://doi.org/10.1079/095442200108729007 DOI: https://doi.org/10.1079/095442200108729007

Zheleva, A. (2012). Electron paramagnetic resonance-oxidative status and antioxidant activity. A monography. Trakia University: Academic Press, Stara Zagora, Bulgaria.

Downloads

Published

2024-05-31

How to Cite

Ojediran, T. K., Alagbe, O. J., Victor, D., & Adewale, E. (2024). Analysis of Kigelia africana (Lam.) Benth. fruit powder’s antioxidant and phytochemical properties. Brazilian Journal of Science, 3(7), 38–49. https://doi.org/10.14295/bjs.v3i7.596

Issue

Section

Agrarian and Biological Sciences