Impact of dietary supplementation of Rhamnus prinoides leaf extract on the growth performance, nutrient retention and intestinal microbial count of “japanese quails”

Authors

DOI:

https://doi.org/10.14295/bjs.v3i5.568

Keywords:

Rhamnus prinoides, phytochemicals, performance, microbial count, quails

Abstract

This research was carried out to investigate the impact of dietary supplementation of Rhamnus prinoides leaf extract (RPL) on the growth performance, nutrient retention and intestinal microbial count of “japanese quails”. 300-2 weeks old “japanese quails” were allocated sixty birds per group and group treatment had four replicates (15 quails per replicate). Corn-soya meal (basal diet) was formulated according to the nutritional needs of birds (NRC, 1994). Quails in group 1(G1) was fed basal diet with no R. prinoides leaf extract, G2, G3, G4 and G5 were fed basal diet with 0.2 mL, 0.4 mL, 0.6 mL and 0.8 mL respectively. Result on the bioactive components in RPL revealed the presence of 15 bioactive compounds with pharmacological properties. Outcome on average daily weight gain showed that G3 (3.20 g), G4 (3.23 g) and G5 (3.24 g) were higher (P < 0.05) relative to G2 (3.06 g) and G1 (2.21 g). These groups also had a better feed conversion ratio (G3, G4 and G5). Average daily feed intake which varied from 703.81-708.33 g were not significantly (P > 0.05) different among the group. Mortality was recorded only among quails in group 1 (2.0%) (P < 0.05). Dry matter, crude protein, ether extract and nitrogen free extracts which varied from 70.12-86.57%, 58.70-68.91% and 50.62-66.05% were higher (P < 0.05) in G3, G4 and G5, intermediate in G2 and lowest in G1. Conversely, crude fibre values were higher in G1 relative to the other groups. Population of Escherichia coli, Salmonella sp. and Staphylococcus sp. count in the gut were lower in G2, G3, G4 and G5 relative to G1 while Lactobacillus sp. count were lowest in G1 (P < 0.05). It was concluded that RPL can be feed to quails up to 0.8 mL/L without causing any negative effect on the growth performance and health status of birds.

References

Abebe, D., Debella, A., & Urga, K. (2003). Medicinal plants and other useful plants of Ethiopia. Bulletin of the Chemical Society of Ethiopia, 2(1), 15-20.

Agubosi, O. C. P., Alexander, J., & Alagbe, J. O. (2022). Influence of dietary inclusion of Sunflower (Helianthus annus) oil on growth performance and oxidative status of broiler chicks. Central Asian Journal of Medical and Natural Sciences, 2(7), 187-195. https://ajird.journalspark.org/index.php/ajird/article/view/7

Alabi, O. J., Malik, A. D., Ng’ambi, J. W., Obaje, P., & Ojo, B. K. (2017). Effect of aqueous Moringa oleifera leaf extracts on the growth performance and carcass characteristics of hubbard broiler chickens. Brazilian Journal of Poultry Science, 19(2), 273-280. https://doi.org/10.1590/1806-9061-2016-0373

Alagbe, J. O (2019). Proximate, mineral and phytochemical analysis of Piliostigma thonningii stems bark and roots. International Journal of Biological, Physical and Chemical Studies, 1(1), 1-7. https://www.al-kindipublisher.com/index.php/ijbpcs/article/view/366

Alagbe, J. O. (2019). Growth response and bacteria count of broiler starter given Delonix regia leaf extract as a natural alternative to antibiotics. Food and Nutrition: Current Research, 2(3), 197-203.

Alagbe, J. O (2020). Proximate, phytochemical and vitamin compositions of Prosopis africana stem bark. Journal of Multidimensional Research & Review, 1(4), 01-12. https://jmrr.org/V1I4/V1I4P01.pdf

Alagbe, J. O., Shittu, M. D., & Ojo, E. A. (2020). Prospect of leaf extracts on the performance and blood profile of monogastric - A review. International Journal of Integrated Education, 3(7), 122-127.

Alagbe, J. O., Shittu, M. D., & Ushie, F. T. (2021). GC-MS analysis of methanolic stem bark extract of Zollingeriana indigofera. Asian Journal of Advances in Research, 4(1), 1265-1267. http://archives.articleproms.com/id/eprint/2064

Alagbe, J. O (2021). Dietary supplementation of Rauvolfia vomitoria root extract as a phytogenic feed additive in growing rabbit diets: Growth performance and caecal microbial population. Concept in Dairy and Veterinary Sciences, 4(2), 409-416. http://dx.doi.org/10.32474/CDVS.2021.04.000184

Alagbe, J. O., & Ushie, F. T. (2022). Growth performance of broiler chicks fed diets containing different levels of aqueous Citrus aurantium stem bark extracts. Discovery, 58(319), 735-741. https://www.discoveryjournals.org/discovery/current_issue/v58/n319/A7.pdf?

Alagbe, J. O., Zubairu, H., Adedeji, O. M., Bamigboye, S., & Dora, A. (2022). Influence of Juniperus thurifera root extract on the nutrient digestibility and caecal microbial count of growing rabbits. Web of Synergy: International Interdisciplinary Research Journal, 1(1), 5-17.

Alagbe, J. O., Shittu, M. D., Ramalan, S. N., Tanimomo, K. B., & Adekunle, D. A. (2022). Growth performance, semen quality characteristics and hormonal profile of male rabbit bucks fed Rubia cordifolia root extracts. International Journal of Biological Engineering and Agriculture, 1(1), 1-13. https://journal.jfpublisher.com/index.php/jpb/article/view/213

Alagbe, J. O (2023). Investigating the effects of dietary supplementation Eucalyptus camaldulensis of essential oil on the growth performance, nutrient digestibility and caecal fermentation of weaned rabbits. Research in: Agricultural and Veterinary Sciences, 7(3), 139-148.

Alagbe, J. O (2023). Investigating the effects of dietary supplementation of Eucalyptus camaldulensis essential oil on haemato-biochemical indices, immune response and oxidative stress of weaned rabbits. Matrix Science Pharma, 7(3), 103-108. DOI: 10.4103/mtsp.mtsp_17_23

Alagbe, O. J. (2023). Impact of dietary supplementation of Carica papaya essential oil on the blood chemistry of broiler chickens. Food Science & Applied Microbiology Reports, 2(2), 58-67. https://doi.org/10.61363/meqmwj94

Alagbe, O. J., Oluchi, C. P. A., Rufus, A. O., Taiwo, O. A., Adegoke, E. A., & Emiola, I. A. (2023). Haemato-biochemical indices and intestinal microbial population of broiler chickens fed diet supplemented with Prosopis africana (African mesquite) essential oil. Brazilian Journal of Science, 2(9), 98-110. https://doi.org/10.14295/bjs.v2i9.390

Alagbe, O. J. (2024). Novel phytogenics’ impact on weaned pigs growth performance, haematology and serum biochemical indicators. Black Sea Journal of Agriculture, 7(2), 82-89. https://doi.org/10.47115/bsagriculture.1328386

Amabye, T. G. (2015). Evaluation of phytochemical, chemical composition, antioxidant and antimicrobial screening parameters of Rhamnus prinoides (Gesho) available in the market of Mekelle, Tigray, Ethiopia. Natural Products Chemistry & Research, 4(1), 1-6. DOI: 10.4172/2329-6836.1000198

Anorue, D. N., Ubong, F., & Alagbe, O. J. (2023). Investigating the effects of pawpaw (Carica papaya) essential oil dietary supplementation on the growth performance and carcass characteristics of broilers. Research in: Agricultural and Veterinary Sciences, 7(3), 164-174. http://jomardpublishing.com/UploadFiles/Files/journals/RV/V7N3/Anorue_et_al.pdf

Asmare, T., Yilkal, B., Mekuannint, T., & Yibeltal, A. (2018). Traditional medicinal plants used to treat maternal and child health illnesses in Ethiopia: An ethno-botanical approach. Journal of Traditional Medicine and Clinical Naturopathy, 7(3), 1-10. DOI: 10.4172/2573-4555.1000277

Awa, E. P., Ibrahim, S., & Ameh, D. A. (2012). GC/MS analysis and antimicrobial activity of dimethyl ether fraction of methanolic extract from

stem bark of Annona senegalensis Pers. International Journal of Phamaceutical Sciences and Research, 3(11), 4213-4218. https://citeseerx.ist.psu.edu/document?repid=rep1&type=pdf&doi=92702a6940db651ac2910826bd0355ee11dad4ce

Bazie, S., Ayalew, A., & Woldetsadik, K. (2014). Antifungal activity of some plant extracts against Colletorichum musae the cause of post-harvest banana Antracrose. Journal of Plant Pathology and Microbiology, 5(2), 1-4. http://dx.doi.org/10.4172/2157-7471.1000226

Berhanu, A. (2014). Microbial profile of Tella and the role of gesho (Rhamnus prinoides) as bittering and antimicrobial agent in traditional Tella (Beer) production. International Food Research Journal, 21(1), 357-365. http://ifrj.upm.edu.my/21%20(01)%202014/51%20IFRJ%2021%20(01)%202014%20%20Berhanu%20012.pdf

Bitew, H., Gebregergs, H., Tuem, K. B., & Yeshak, M. Y. (2019). Ethiopian medicinal plants traditionally used for wound treatment: a systematic review. Ethiopian Journal of Health Development, 33(2), 103-112. https://www.ajol.info/index.php/ejhd/article/view/188853

Bosire, K. O. (2003). An investigation of a traditional herbal therapy used to treat malaria in Kisii [MSc Thesis] University of Nairobi, Nairobi, Kenya. http://erepository.uonbi.ac.ke:8080/xmlui/handle/11295/24728

Caroline, G. V. (2022). Improving feed efficiency and gut integrity of E. coli challenged weaned pig's. International Pig Magazine, 36(3), 7-8. https://www.cabidigitallibrary.org/doi/full/10.5555/20220444753

Devendran, G., & Balasubramanian, U. (2011). Qualitative phytochemical screening and GC-MS analysis of Ocimum santum L. leaves. Asian Journal of Plant Science and Research, 1(4), 44-48. https://hal.science/hal-03699060/

Doughari, M., Hamuel, J., & Saa-Aondo, M. (2021). Phytochemical analysis of crude methanol extracts and antimicrobial activity of n-hexane fractions of methanol seed and pod extracts of Prosopis africana on some selected microoganisms. Pharmacology Online, 21(2), 121-137. https://pharmacologyonline.silae.it/files/archives/2021/vol2/PhOL_2021_2_A016_Doughari.pdf

Dzoyem, J. P., Aro, A. O., McGaw, L. J., & Eloff, J. N. (2016). Antimycobacterial activity against different pathogens and selectivity index of fourteen medicinal plants used in southern Africa to treat tuberculosis and respiratory ailments. South African Journal of Botany, 102, 70-74. https://doi.org/10.1016/j.sajb.2015.08.002

Esonu, B. O., Iheukwumere, F. C., Emenalom, O. O., & Etuk, B. B. (2002). Performance, nutrient utilization and organ characteristics of broilers fed Microdemis puberula leaf. Livestock Research and Rural Development, 14(6), 11-16. https://www.lrrd.cipav.org.co/lrrd14/6/eson146.htm

Gebru, M. (2010). Phytochemical and antiplasmodial investigation of Rhamnus prinoides and Kniphofia foliosa [MSc Thesis]. University of Nairobi, Nairobi, Kenya.

Giday, M., Asfaw, Z., & Woldu, Z. (2010). Ethnomedicinal study of plants used by Sheko ethnic group of Ethiopia. Journal of Ethnopharmacology, 132(1), 75-85. https://doi.org/10.1016/j.jep.2010.07.046

Goliomytis, M., Tsoureki, D., Simitzis, P. E., Charismiadou, M. A., Hager-Theodorides, A. L., & Deligeorgis, S. G. (2014). The effects of quercetin dietary supplementation on broiler growth performance, meat quality, and oxidative stability. Poultry Science, 93(8), 1957-1962. https://doi.org/10.3382/ps.2013-03585

Mangrove-Abayomi, O. E., Kenneth, E., & Mkaparu, K. I. (2014). Chemometric profiling of methanolic leaf extract of Cinddoscolus aconitifoliuus (Euphorbiaceae) using UV-VIS, FTIR and GC-MS techniques. Peak Journal of Medicinal Plant Research, 2(1), 6-12.

Megersa, M., Asfaw, Z., Kelbessa, E., Beyene, A., & Woldeab, B. (2013). An ethnobotanical study of medicinal plants in Wayu Tuka district, east Welega zone of oromia regional state, West Ethiopia. Journal of Ethnobiology and Ethnomedicine, 9(1), 1-18. https://doi.org/10.1186/1746-4269-9-68

Musa, B., Alagbe, J. O., Betty, A. M., & Omokore, E. A. (2020). Growth performance, caeca microbial population and immune response of broiler chicks fed aqueous extract of Balanites aegyptiaca and Alchornea cordifolia stem bark mixture. United Journal for Research and Technology, 1(11), 13-21. https://uijrt.com/articles/v1/i11/UIJRTV1I110002.pdf

Namkeleja, H. S., Tarimo, N. T. C., & Ndakidemi, P. A. (2014). Allelopathic effects of Argemone mexicana to growth of native plant species. American Journal of Plant Sciences, 5(9), 1336-1344. http://www.scirp.org/journal/PaperInformation.aspx?PaperID=44882

Nester, M. T., Annderson, D. G., Roberts, J. C. E., & Pearlsall, N. N. (2002). Microbiology - A human perspective genitourinary infections and antimicrobial medications. 3rd Ed. McGraw Hill. Madrid, 21-25; 496-664.

Nigussie, G., Alemu, M., Ibrahim, F., Werede, Y., Tegegn, M., Neway, S., & Endale, M. (2021). Phytochemicals, traditional uses and pharmacological activity of Rhamnus prinoides: A review. International Journal of Secondary Metabolite, 8(2), 136-151. https://www.cabidigitallibrary.org/doi/full/10.5555/20210371881

Oloruntola, O. D., Ayodele, S. O., Agbede, J. O., Oloruntola, D. A., Ogunsipe, M. H., & Omoniyi, I. S. (2016). Effect of Alchornea cordifolia leaf meal and enzyme supplementation on growth, haematological, immunostimulatory and serum biochemical response of rabbits. Asian Journal of Biological and Life Sciences, 5(2), 190-195.

Sen, A., & Batra, A. (2012). Evaluation of antimicrobial activity of different solvent extracts of medicinal plant: Melia azedarach L. International Journal of Current Pharmaceutical Research, 4(2), 67-73.

Sharma, S., Alagbe, O. J., Xing, L., Ram, S., & Amita, K. (2022). Comparative analysis of ethanolic Juniperus thurifera leaf, stem bark and root extract using gas chromatography and mass spectroemetry. International Journal of Agriculture and Animal Production, 2(6), 18-27. https://doi.org/10.55529/ijaap.26.18.27

Shittu, M. D., & Alagbe, J. O. (2020). Phyto-nutritional profiles of broom weed (Sida acuta) leaf extract. Annals of Clinical Toxicology, 3(2). https://pdfs.semanticscholar.org/4389/c3e04854f79f4a617939de0447197b556040.pdf

Shittu, M. D., Alagbe, J. O., Adejumo, D. O., Ademola, S. G., Abiola, A. O., Samson, B. O., & Ushie, F. T. (2021). Productive performance, caeca microbial population and immune-modulatory activity of broiler chicks fed different levels Sida acuta leaf extract in replacement of antibiotics. Journal of Multidimensional Research & Review, 2(4), 34-42. https://jmrr.org/V2I4/V2I4P05.pdf

Subavathy, P., & Thilaga, R. D. (2015). GC-MS analysis of bioactive compounds from whole body of methanolic extract of Cypraea arabica. World Journal of Pharmaceutical Research, 5(3), 800-806. https://wjpr.s3.ap-south-1.amazonaws.com/article_issue/1456804218.pdf

Vadivel, B., Veluchamy, B., & Arjunan, S. (2015). GC/MS analysis of leaf and bark extract of Moringa concanensis a siddha medicinal plant of South India. European Journal of Biotechnology and Bioscience, 3(12), 57-61.

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Published

2024-04-24

How to Cite

Alagbe, J. O. (2024). Impact of dietary supplementation of Rhamnus prinoides leaf extract on the growth performance, nutrient retention and intestinal microbial count of “japanese quails” . Brazilian Journal of Science, 3(5), 40–50. https://doi.org/10.14295/bjs.v3i5.568

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Agrarian and Biological Sciences