Phytochemical prospection of the floral extract of Ouratea spectabilis (Mart.) Engl. (Ochnaceae)
DOI:
https://doi.org/10.14295/bjs.v1i8.162Keywords:
Ouratea genus, Phenolics, Alkaloids, Antioxidant activity, Hemolytic activityAbstract
Ouratea spectabilis is a tree species belonging to the genus Ouratea and the family Ochnaceae. This study aimed to report the first report on the phytochemical constitution of the floral extract of O. spectabilis. Flowers were collected from individuals of O. spectabilis in a Cerrado area in Rio Verde, Goiás, Brasil in 2022. The extract was produced from 150g of floral organ. The extract yield was determined by the difference in mass, visual analysis, crystallinity and homogeneity were performed by the organoleptic method, pH and electrical conductivity were determined using a pHmeter and conductivitymeter both digital. The qualitative phytochemical profile was performed for several groups of phytomolecules of the special metabolism of this plant using specific reagents. The floral extract yield was 11.81%, several phytochemical groups were observed, such as phenolics, saponins, flavonoids, steroids, triterpenoids, organic acids, reducing sugars, aromatic compounds, alkaloids and amino acids. The floral extract of Ouratea spectabilis showed high potential for future work on chemical quantification and assays with different biological models.
References
Alves-Silva, E. & Del-Claro, K. (2016). Wasps are better plant-guards than ants in the extrafloral nectaried shrub Ouratea spectabilis (Ochnaceae). Sociobiology, 63(1), 705-711. https://doi.org/10.13102/sociobiology.v63i1.908 DOI: https://doi.org/10.13102/sociobiology.v63i1.908
Araújo, M. F., Santos, G. B., Cavalcanti, J. F., Pereira, F. S., Mendes, G. S., Werle, A. A., Romanos, M. T. V. & Carvalho, M. G. (2011). Proposed active compounds from Ouratea parviflora. Journal of Medicinal Plants Research, 5(12), 2489-2493. https://doi.org/10.5897/JMPR.9001054
Bagla, V. P., McGraw, L. J. & Eloff, J. N. (2012). The antiviral activity of six South African plants traditionally used against infections in ethnoveterinary medicine. Veterinary Microbiolog, 155(2-4), 198-206. https://doi.org/10.1016/j.vetmic.2011.09.015 DOI: https://doi.org/10.1016/j.vetmic.2011.09.015
Brandão, G. C., Kroon, E. G., Santos, J. R., Stehmann, J. R., Lombardi, J. A. & Oliveira A. B. J. (2011). Antiviral activity of plants occurring in the State of Minas Gerais (Brazil): Part III. Chemical & Pharmaceutical Research, 3, 223-236.
Braga, F. C., Wagner, H., Lombardi, J. A. & Oliveira, A. B. (2000). Screening Brazilian plant species for in vitro inhibition of 5-lipoxygenase. Phytomedicine, 6(6), 447-452. https://doi.org/10.1016/S0944-7113(00)80073-2 DOI: https://doi.org/10.1016/S0944-7113(00)80073-2
Campana, P. R. V., Mansur, D. S., Gusman, G. S., Ferreira, D., Teixeira, M. M., Braga, F. C. (2015). Phytotherapy Research, 29(10), 1509-1515. https://doi.org/10.1002/ptr.5401 DOI: https://doi.org/10.1002/ptr.5401
Chacon, R.G., 2011. Ochnaceae nos estados de Goiás e Tocantins, Brasil. Brasilia, 122p. Dissertação de mestrado, Universidade de Brasília. Programa de Pós-Graduação em Botânica, UnB, Brasília, Distrito Federal, Brasil, 2011. 138 p.
Carbonari, K. A., Ferreira, E. A., Rebello, J. M., Felipe, K. B., Rossi, M. H., Felício, J. D., Filho, D. W., Yunes, R. A. Pedrosa, R. C. (2006). Free-radical scavenging by Ouratea parviflora in experimentally-induced liver injuries. Redox Report, 11(3), 124-130. https://doi.org/10.1179/135100006X116619 DOI: https://doi.org/10.1179/135100006X116619
Carbonezi, C. A., Hamerski, L., Gunatilaka, A. A. L., Cavalheiro, A., Castro-Gamboa, I., Silva, D. H. S., Furlan, M., Young, M. C. M., Lopes, M. N. & Bolzani, V. S. (2007). Bioactive flavone dimers from Ouratea multiflora (Ochnaceae). Revista Brasileira de Farmacognosia, 17(3), 319-324. https://doi.org/10.1590/S0102-695X2007000300003 DOI: https://doi.org/10.1590/S0102-695X2007000300003
Carvalho, M.G., Carvalho, G.J.A., Braz-Filho, R., 2000. Chemical constituents from Ouratea floribunda: complete 1H and 13C NMR assignments of atranorin and its new acetyl derivative. Journal of the Brazilian Chemical Society, 11(2), 143-147. https://doi.org/10.1590/S0103-50532000000200007 DOI: https://doi.org/10.1590/S0103-50532000000200007
Côrtes, S. F., Valadares, Y. M., Oliveira, A. B., Lemos, V. S., Barbosa, M. P. T. & Braga, F. C. (2002). Mechanism of endothelium-dependent vasodilation induced by a proanthocyanidin-rich fraction from Ouratea semiserrata. Planta Medica, 68(5), 412-415. 10.1055/s-2002-32079 DOI: https://doi.org/10.1055/s-2002-32079
Daniel, J. F. S., Alves, C. C. F., Grivicich, I., Rocha, A. B. & Carvalho, M. G. (2007). Anti-tumour activity of biflavonoids from Ouratea and Luxemburgia on human cancer cell lines. Indian Journal of Pharmacology, 39(4), 184-186. https://www.ijp-online.com/text.asp?2007/39/4/184/36536 DOI: https://doi.org/10.4103/0253-7613.36536
Estevam, C. S., Oliveira, F. M., Conserva, L. M., Lima, L. F. C. O., Barros, E. C. P., Barros, A. C. P., Rocha, E. M. M. & Andrade, E. H. A. (2005). Constituintes quimicos e avaliacão preliminar in-vivo da atividade antimalarica de Ouratea nítida Aubl (Ochnaceae). Revista Brasileira de Farmacognosia, 15(3), 195-198. https://doi.org/10.1590/S0102-695X2005000300005 DOI: https://doi.org/10.1590/S0102-695X2005000300005
Fidelis, Q. C., Ribeiro, T. A. N., Araújo, M. F. & Carvalho, M. G. (2014). Ouratea genus: chemical and pharmacological aspects. Revista Brasileira de Farmacognosia, 24(1), 1-19. 10.1590/0102-695X20142413361
Felício, J., Gonçalez, E., Braggio, M. M., Costantino, L., Albasini, A. & Lins, A. P. (1995). Inhibition of lens aldose reductase by biflavones from Ouratea spectabilis. Planta Medica, 61(3), 217-220. 10.1055/s-2006-958059 DOI: https://doi.org/10.1055/s-2006-958059
Fidelis, Q. C., Ribeiro, T. A. N., Araújo, M. F., de Carvalho, M. G. (2014). Ouratea genus: chemical and pharmacological aspects. Revista Brasileira de Farmacognosia, 24(1), 1-19. https://doi.org/10.1590/0102-695X20142413361 DOI: https://doi.org/10.1590/0102-695X20142413361
Grynberg, N. F., Carvalho, M. G., Velandia, J. R., Oliveira, M. C., Moreira, I. C., Braz-Filho, R. & Echevarria, A. (2002). DNA topoisomerase inhibitors: biflavonoids from Ouratea species. Brazilian Journal of Medicinal Biology Research, 35(7), 819-822. https://doi.org/10.1590/S0100-879X2002000700009 DOI: https://doi.org/10.1590/S0100-879X2002000700009
Koomson, D. A., Kwakye, B. D., Darkwah, W. K., Odum, B., Asante, M. & Aidoo, G. (2018). Phytochemical constituients, total saponins, alkaloids, flavonoids and vitamin C contents of ethanol extracts of five Solanum torvum fruits. Farmacognosy Journal, 10(5), 946-950. http://dx.doi.org/10.5530/pj.2018.5.160 DOI: https://doi.org/10.5530/pj.2018.5.160
Madike, L. N., Takaidza, S. & Pillay, M. (2017). Preliminary phytochemical screening of crude extracts from the leaves, stems, and roots of Tulbaghia violacea. International Journal of Pharmacognosy and Phytochemical Research, 9(10), 1300-1308. https://doi.org/10.25258/phyto.v9i10.10453 DOI: https://doi.org/10.25258/phyto.v9i10.10453
Matos, F. J. (1997). Introdução à fitoquímica experimental. Fortaleza, Ceará, Ed., UFC, 2ª Ed.
Mecina, G. F., Santos, V. H. M., Dokkedal, A. L., Saldanha, L. L., Silva, L. P. & Silva, R. M. G. (2014). Phytotoxicity of extracts and fractions of Ouratea spectabilis (Mart. ex Engl.) Engl. (Ochnaceae). South African Journal of Botany, 95, 174-180. https://doi.org/10.1016/j.sajb.2014.10.002 DOI: https://doi.org/10.1016/j.sajb.2014.10.002
Mehdi, M. A. H., Alarabi, F. Y. S., Farooqui, M. & Pradhan, V. (2019). Phytochemical screening and antiamebic studies of Tamarindus indica of leaves extract. Asian Journal of Pharmaceutical and Clinical Research, 12(2), 507-512. DOI: https://doi.org/10.22159/ajpcr.2019.v12i2.29684
Menezes Filho, A. C. P., Ventura, M. V. A., Alves, I., Taques, A. S., Batista-Ventura, H. R. F., Castro, C. F. S., Teixeira, M. B. & Soares, F. A. L. (2022). Phytochemical prospection, total flavonoids and total phenolics and antioxidant activity of the mushroom extract Scleroderma verrucosum (Bull.) Pers. Brazilian Journal of Science, 1(1), 1-7. https://www.brazilianjournalofscience.com.br/revista/article/view/2/1
Menezes Filho, A. C. P., Santos, M. C. & Castro, C. F. D. (2021). Estudo fitoquímico, físico-químico e bioativo do extrato hidroetanólico floral de Ouratea lancifolia R. G. Chacon & K. Yamam. (Ochnaceae). Scientia Naturalis, 3(1), 21-40. https://revistas.ufac.br/index.php/SciNat/article/view/4361 DOI: https://doi.org/10.5935/2595-4407/rac.immes.v3n2p59-71
Mbing, J. N., Enguehard-Gueiffier, C., Atchade, A. T., Allouchi, H., Gangoue-Pieboji, J., Mbafor, J. T., Tih, R. G., Pothier, J.,
Pegnyemb, D. E. & Gueiffier, A. (2006). Two biflavonoids from Ouratea nigroviolacea. Phytochemistry, 67(24), 2666-2670. https://doi.org/10.1016/j.phytochem.2006.07.027 DOI: https://doi.org/10.1016/j.phytochem.2006.07.027
Montesinos, D. & Oliveira, P. (2014). Reproductive ecology of buzz-pollinated Ouratea spectabilis trees (Ochnaceae) in Brazilian Cerrados. Web Ecology, 14(1), 79-84. https://doi.org/10.5194/we-14-79-2014 DOI: https://doi.org/10.5194/we-14-79-2014
Oliveira, M. M., Sampaio, M. P., Simon, F., Gilbert, B. & Mors, W. B. (1972). Anti-tumour activity of condensed flavanols. Anais da Academia Brasileira de Ciências, 44, 41-44.
Paulo, M. Q., Lima, e. o., Maia, R. F. & Xavier Filho, L. (1986). Atividade antimicrobiana do óleo dos frutos de Ouratea parviflora Baill (Ocnaceae). CCS – Ciência, Cultura e Saúde, 8(3), 19-21. http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7433508
Pegnyemb, D. E., Mbing, J. N., Atchade, A. T., Tih, R.G., Sondengam, B. L., Blond, A. & Bodo, B. (2005). Antimicrobial biflavonoids from the aerial parts of Ouratea sulcata. Phytochemistry, 66(16), 1922-1926. https://doi.org/10.1016/j.phytochem.2005.06.017 DOI: https://doi.org/10.1016/j.phytochem.2005.06.017
Rocha, M. P., Campana, P. R. V., Pádua, R. M., Souza Filho, J. D., Ferreira, D. & Braga, F. C. (2020). (3,3”)-Linked biflavanones from Ouratea spectabilis and their effects on the release of proinflammatory cytokines in THP-1 cells. Journal of Natural Products, 83(6), 1891-1898. https://doi.org/10.1021/acs.jnatprod.0c00074 DOI: https://doi.org/10.1021/acs.jnatprod.0c00074
Roming, T. L., Weber, N. D., Murray, B. K., North, J. A., Wood, S. G., Hughes, B. G. & Gates, R. G. (1992). Antiviral activity of Panamanian plant extracts. Phytotherapy Research, 6(1), 38-43. https://doi.org/10.1002/ptr.2650060110 DOI: https://doi.org/10.1002/ptr.2650060110
Valadares, Y. M., Oliveira, A. B., Cortes, S. F., Lombardi, J. A. & Braga, F. C. (2003). Atividade vasodilatadora in-vitro de especies de Ouratea (Ochnaceae) e de fracoes de Ouratea semisserrata (Mart.) Engl. Brazilian Journal Pharmacy Science, 39(1), 83-91. https://doi.org/10.1590/S1516-93322003000100009 DOI: https://doi.org/10.1590/S1516-93322003000100009
Velandia, J. R., de Carvalho, M. G., Braz-Filho, R. & Werle, A. A. (2002). Biflavonoids and a glucopyranoside derivative from Ouratea semiserrata. Phytochemical Analysis, 13(5), 283-292. https://doi.org/10.1002/pca.656 DOI: https://doi.org/10.1002/pca.656
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Copyright (c) 2022 Antonio Carlos Pereira de Menezes Filho, Matheus Vinicius Abadia Ventura, Carlos Frederico de Souza Castro, Marconi Batista Teixeira, Frederico Antônio Loureiro Soares, Hellen Regina Fernandes Batista-Ventura, Aparecida Sofia Taques
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