Phytochemical prospection, total flavonoids and total phenolic and antioxidant activity of the mushroom extract Scleroderma verrucosum (Bull.) Pers

Authors

DOI:

https://doi.org/10.14295/bjs.v1i1.2

Keywords:

Scleroderma genus, Antioxidant activity, Flavonoids content, Total phenols, Sclerodermataceae

Abstract

Scleroderma verrucosum is a species of mushroom belonging to the family Sclerodermataceae found in the Americas and Europe. This study aimed to evaluate the 70% ethanol extract of the vegetative part of the mushroom S. verrucosum for qualitative phytochemical constituents and total content of flavonoids and phenolics, and the DPPH free radical reduction activity. Mushroom extract was prepared in 70% ethanol solution. Qualitative phytochemical assay was performed for different groups using colorimetric reagents. The contents of total flavonoids and phenolics, and antioxidant activity in reducing the DPPH free radical were quantitatively determined. The positive presence of alkaloids, flavonoids, phenolics, triterpenoids, steroids, tannins, organic acids, reducing sugars, aromatic compounds and carboxylic acids were observed. The extract exhibited total flavonoid content = 60.14 QE 100 g-1, for total phenolics = 309.14 GAE 100 g-1 and free radical reduction of = 5.97 µg mL-1. Mushroom extract Scleroderma verrucosum demonstrated the presence of several medicinal important phytochemical groups as well as total flavonoid and phenolic content that exhibit the potential for antioxidant activity.

References

Ben-Shabat, S., Yarmolinsky, L., Porat, D., Dahan, A. (2020). Antiviral effect of phytochemicals from medicinal plants: Applications and drug delivery strategies. Drug Delivery and Translational Research, 10, 354-376. https://doi.org/10.1007/s13346-019- 00691-6 DOI: https://doi.org/10.1007/s13346-019-00691-6

Cortez, V. G., Baseia, I. G., Silveira, R. M. B. (2011). Gasteroid mycobiota of Rio Grande do Sul, Brazil: Boletales. Journal of Yeast and Fungal Research, 2(4), 44-52.

De Silva, G. O., Abeysundara, A. T., Aponso, M. M. W. Extraction methods, qualitative and quantitative techniques for screening of phytochemicals from plants. American Journal of Essential Oils and Natural Products, 5(2), 29-32.

Erguven, M., Yilmaz, O., Deveci, M., Aksu, N., Dursun, F., Pelit, M., Cebeci, N. (2007). Mushroom poisoning. Indian Journal of Pediatrics, 74, 847-852. https://doi.org/10.1007/s12098-007- 0151-6 DOI: https://doi.org/10.1007/s12098-007-0151-6

Furlani, R. P. Z., Godoy, H. T. (2007). Valor nutricional de cogumelos comestíveis. Food Science and Technology, 27(1), 154-157. https://doi.org/10.1590/S0101-20612007000100027 DOI: https://doi.org/10.1590/S0101-20612007000100027

Gonzalez, A. G., Barrera, J. B., Marante, F. J. T. (1983). The steroids and fatty acids of the basidiomycete Scleroderma polyrhizum. Phytochemistry, 22(4), 1049-1050. https://doi.org/10.1016/0031-9422(83)85062-6 DOI: https://doi.org/10.1016/0031-9422(83)85062-6

Guzmán, G., Cortés-Pérez, A., Guzmán-Dávalos, L., Ramírez-Guillén, F., Sánchez-Jácome, M. R. (2013). An emendation of Scleroderma, new records, and review of the known species in Mexico. Revista Mexicana de Biodiversidad, 84, S173-S191. https://doi.org/10.7550/rmb.31979

Guzmán, G. (1967). Taxonomía del género Scleroderma Pers. emend. Fr. Ciencia, 25, 195-208.

Guzmán, G., Cortóes-Pérez, A., Guzmán-Dávalos, L. et al. An emendation of Scleroderma, new redords, and review of the known species in Mexico. Revista Mexicana de Biodiversid, 84, 173-191. https://doi.org/10.7550/rmb.31979 DOI: https://doi.org/10.7550/rmb.31979

Jia, H., Li, S., Wu, L., Li, S., Sharma, V. K., Yan, B. (2020). Cytotoxic free radicals on air-borne soot particles generated by burning wood or low-maturity coals. Environmental Science & Technology, 54(9), 5608-5618. https://doi.org/10.1021/acs.est.9b06395 DOI: https://doi.org/10.1021/acs.est.9b06395

Jung, N. C., Tamai, Y. (2012). Anatomical observation of polyphenol changes in epidermal cells during the development of Quercus acutissima – Scleroderma verrucosum ectomycorrhizae. Trees, 26, 301-310. 10.1007/s00468-011- 0592-4 DOI: https://doi.org/10.1007/s00468-011-0592-4

Łopusiewicz, Ł. (2018). Scleroderma citrinum melanin: isolation, purification, spectroscopic studies with characterization of antioxidant, antibacterial and light barrier properties. World Scientific News, 94(2), 115-130.

Morandini, L. M. B., Neto, A. T., Pedroso, M., Antoniolli, Z. I. et al. (2016). Lanostane-type triterpenes from the fungal endophyte Scleroderma UFSMSc1 (Persoon) Fries. Bioorganic & Medicinal Chemistry Letters, 26(4), 1173-1176. DOI: https://doi.org/10.1016/j.bmcl.2016.01.044

Montagner, D. F. (2014). Caracterização do gênero Scleroderma (Basidiomycotina) associado a povoamentos florestais exóticos no bioma Pampa, Brasil. Dissertação (Mestrado em Agrobiologia), Universidade Federal de Santa Maria, Santa Maria, RS, Brasil. 50 f., 2014.

Nouhra, E. R., Caffot, M. L. H., Pastor, N., Crespo, E. M. (2012). The species of Scleroderma from Argentina, including a new species from the Nothofagus forest. Mycologia, 104(1), 488- 495. DOI: https://doi.org/10.3852/11-082

Orsine, J. V. C., Brito, L. M., Novaes, M. R. C. G. (2012). Cogumelos comestíveis: uso, conservação, características nutricionais e farmacológicas. Clinical & Biomedical Research, 32(4), 452- 460.

Pakkirisamy, M., Kalakandan, S. K., Ravichandran, K. (2017). Phytochemical screening, GC-MS, FT-IR analysis of methanolic extract of Curcuma caesia Roxb (Black Turmeric). Pharmacognosy Journal, 9(6), 952-956. 10.5530/pj.2017.6.149 DOI: https://doi.org/10.5530/pj.2017.6.149

Pinzón-Osorio, C. A., Pinzón-Osorio, J. (2018). Primer registro de Scleroderma bovista (Boletales, Sclerodermataceae) para Colombia. Revista Peruana de Biología, 25(4), 457-462. http://dx.doi.org/10.15381/rpb.v25i4.14550 DOI: https://doi.org/10.15381/rpb.v25i4.14550

Selim, K. A., Elkhateeb, W. A., Tawila, A. M., El-Beih, A. A., AbdelRahman, T. M., El-Diwany, A. I., Ahmed, E. F. (2018). Antiviral and antioxidant potential of fungal endophytes of Egyptian medicinal plants. Fermatation, 4(3), 49. https://doi.org/10.3390/fermentation4030049 DOI: https://doi.org/10.3390/fermentation4030049

Sembiring, E. N., Elya, B., Sauriasari, R. (2018). Phytochemical screening, total flavonoid and total phenolic content and antioxidant activity of different parts of Caesalpinia bonduc (L.) Roxb. Pharmacognosy Journal, 10(1), 123-127. DOI: https://doi.org/10.5530/pj.2018.1.22

Sulzbacher, M. A., Grebenc, T., Jacques, R. J. S., Antoniolli, Z. I. (2013). Ectomycorrhizal fungi from southern Brazil – a literaturebased review, their origin and potential hosts. Mycosphere, 4(1), 61-95. DOI: https://doi.org/10.5943/mycosphere/4/1/5

Teles, A. M., Everton, G. O., Mouchrek, A. N., Tellis, C. J. M., Bezerra, G. F. B., Souza, F. A., Azevedo-Santos, A. P. S., Nascimento, M. D. S. B. (2020). Triagem fitoquímica de extrato obtido do fungo Penicillium purpurogenum advindo de ambiente marinho poluído do Maranhão. Research, Society and Development, 9(8), e539986109. DOI: https://doi.org/10.33448/rsd-v9i8.6109

Vrkoč, J., Buděšínský, M., Dolejš, L. (1976). Constituents of the basidiomycete Scleroderma auratium. Phytochemistry, 15(11), 1782-1784. https://doi.org/10.1016/S0031-9422(00)97486-7 DOI: https://doi.org/10.1016/S0031-9422(00)97486-7

White, J., Weinstein, S. A., Haro, L. De., Bédry, R., Schaper, A., Rumack, B. H., Zilker, T. (2019). Mushroom poisoning: A proposed new clinical classification. Toxicon, 157, 53-65. https://doi.org/10.1016/j.toxicon.2018.11.007 DOI: https://doi.org/10.1016/j.toxicon.2018.11.007

Yayli, N., Yilmaz, N., Ocak, M., Sevim, A. (2007). Essential oil compositions of four mushrooms: Scleroderma verrucosum, Cortinarius infractus, Hypholama capnoides and Hypholama fasciculares from Turkey. Asian Journal of Chemistry, 19(5), 4102-4106.

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Published

2022-01-01

How to Cite

Menezes Filho, A. C. P. de, Ventura, M. V. A., Alves, I., Taques, A. S., Batista-Ventura, H. R. F., Castro, C. F. de S., Teixeira, M. B., & Soares, F. A. L. (2022). Phytochemical prospection, total flavonoids and total phenolic and antioxidant activity of the mushroom extract Scleroderma verrucosum (Bull.) Pers. Brazilian Journal of Science, 1(1), 1–7. https://doi.org/10.14295/bjs.v1i1.2

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Section

Agrarian and Biological Sciences